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igg2a mabs isotype control  (Bio X Cell)


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    Bio X Cell igg2a mabs isotype control
    Igg2a Mabs Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg2a+isotype+control/InVivoMAb+rat+IgG2a+isotype+control%2C+anti-trinitrophenol/pmc13650256-56-33-42
    Average 98 stars, based on 1800 article reviews
    igg2a mabs isotype control - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Control:

    Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy
    Article Snippet: Rat anti-mouse PD-1 (Clone RMP1-14) , BioXCell , Cat# BX-BE0146; RRID: AB_10949053. .. Rat IgG2a isotype control (Clone 2A3) , BioXCell , Cat# BX-BE0089; RRID: AB_1107769. ..

    Article Title: Systemic Cysteine Elevation Sustains T-Cell Activation to Potentiate PD-1 Blockade
    Article Snippet: .. Rat IgG2a isotype control (5 mg/kg, Bioxcell, BP0089) or anti-mouse PD-1 (5 mg/kg, Bioxcell, BP0146) were intraperitoneally injected twice weekly in both the KPC and KC PDAC models. ..

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To activate in vivo NK cells, C57BL/6J wild type mice were injected i.p. with a neutralizing rat anti-mouse NKG2A/C/E monoclonal antibody (200g/mouse, BioXCell BE0321, clone 20D5) or with a rat IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: .. A neutralizing anti-PLVAP antibody (clone MECA-32, BioXCell, BE0200) or a rat IgG2a isotype control (BioXCell, BE0089) was diluted to a concentration of 2.11 mg/ml in a 0.9% sodium chloride solution (Millipore Sigma, S8776). .. Osmotic pumps (ALZET, model 2002) were filled with the diluted antibodies according to the manufacturer’s protocol, and then surgically implanted subcutaneously into DIO mice at 20 weeks-of-age.

    Article Title: Targeting Mettl8-Tcf1 axis promotes CD8 + T PEX differentiation and antitumor immunity
    Article Snippet: .. Anti–PD-1 treatments involved i.p. administration of 200 μg per mouse of anti–PD-1 antibody (clone RMP1-14, cat. no. A2122; Selleckchem) or rat IgG2a isotype control (clone 2A3, cat. no. BE0089; Bio X Cell) in 200 μL PBS every 2 days from day 11 to 17 or day 12 to 18 after inoculation. ..

    Article Title: Targeting Mettl8-Tcf1 axis promotes CD8+ TPEX differentiation and antitumor immunity.
    Article Snippet: .. Anti–PD-1 treatments involved i.p. administration of 200 μg per mouse of anti–PD-1 antibody (clone RMP1-14, cat. no. A2122; Selleckchem) or rat IgG2a isotype control (clone 2A3, cat. no. BE0089; Bio X Cell) in 200 μL PBS every 2 days from day 11 to 17 or day 12 to 18 after inoculation. ..

    Article Title: Leishmania major co-opts IL-7 feedback in monocytes to suppress CD4⁺ T-cell immunity
    Article Snippet: For anti-IL-7/IL-7R application, mice were intraperitoneally (i.p.) injected with 500 μg of anti-mouse IL-7Rα (clone A7R34, Bio X Cell), which is non-depleting ( ; ), and 500 μg of anti-mouse IL7 (clone M25, Bio X Cell) per treatment (Table S4). .. Control mice were i.p. injected with 500 μg of rat IgG2a isotype control (clone 2A3, Bio X Cell) and 500 μg of mouse IgG2b isotype control (clone MPC-11, Bio X Cell) per treatment. .. Where indicated, Tamoxifen (Sigma-Aldrich) was dissolved in olive oil (Sigma-Aldrich) at a concentration of 20 mg/ml and administered by i.p. injection (100 mg/kg) for five consecutive days, either five days before the infection, or between days 11 through 15 p.i.

    Article Title: Macrophages regulate meiotic initiation and germ cell clearance in the developing ovary
    Article Snippet: For Cx3cr1 -creER; Rosa -Tomato experiments, two regimens were used: to label embryonic Cx3cr1 + macrophages, pregnant females were injected with 75 μg/g 4-OHT and 37.5 μg/g progesterone at E12.5; to label early postnatal monocytes and macrophages, Cx3cr1 -creER; Rosa -Tomato pups received 50 μg tamoxifen (TAM, Sigma-Aldrich #T5648) intraperitoneally on P4 and P5. .. To transiently deplete yolk-sac-derived and fetal CSF1R + macrophages, pregnant C57BL/6J females were injected intraperitoneally with 3 mg anti-CSF1R monoclonal antibody (mAb; clone AFS98, Bio X Cell #BP0213) or rat IgG2a isotype control (Bio X Cell #BP0089). ..

    Injection:

    Article Title: Systemic Cysteine Elevation Sustains T-Cell Activation to Potentiate PD-1 Blockade
    Article Snippet: .. Rat IgG2a isotype control (5 mg/kg, Bioxcell, BP0089) or anti-mouse PD-1 (5 mg/kg, Bioxcell, BP0146) were intraperitoneally injected twice weekly in both the KPC and KC PDAC models. ..

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To activate in vivo NK cells, C57BL/6J wild type mice were injected i.p. with a neutralizing rat anti-mouse NKG2A/C/E monoclonal antibody (200g/mouse, BioXCell BE0321, clone 20D5) or with a rat IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Article Title: Leishmania major co-opts IL-7 feedback in monocytes to suppress CD4⁺ T-cell immunity
    Article Snippet: For anti-IL-7/IL-7R application, mice were intraperitoneally (i.p.) injected with 500 μg of anti-mouse IL-7Rα (clone A7R34, Bio X Cell), which is non-depleting ( ; ), and 500 μg of anti-mouse IL7 (clone M25, Bio X Cell) per treatment (Table S4). .. Control mice were i.p. injected with 500 μg of rat IgG2a isotype control (clone 2A3, Bio X Cell) and 500 μg of mouse IgG2b isotype control (clone MPC-11, Bio X Cell) per treatment. .. Where indicated, Tamoxifen (Sigma-Aldrich) was dissolved in olive oil (Sigma-Aldrich) at a concentration of 20 mg/ml and administered by i.p. injection (100 mg/kg) for five consecutive days, either five days before the infection, or between days 11 through 15 p.i.

    Article Title: Macrophages regulate meiotic initiation and germ cell clearance in the developing ovary
    Article Snippet: For Cx3cr1 -creER; Rosa -Tomato experiments, two regimens were used: to label embryonic Cx3cr1 + macrophages, pregnant females were injected with 75 μg/g 4-OHT and 37.5 μg/g progesterone at E12.5; to label early postnatal monocytes and macrophages, Cx3cr1 -creER; Rosa -Tomato pups received 50 μg tamoxifen (TAM, Sigma-Aldrich #T5648) intraperitoneally on P4 and P5. .. To transiently deplete yolk-sac-derived and fetal CSF1R + macrophages, pregnant C57BL/6J females were injected intraperitoneally with 3 mg anti-CSF1R monoclonal antibody (mAb; clone AFS98, Bio X Cell #BP0213) or rat IgG2a isotype control (Bio X Cell #BP0089). ..

    In Vivo:

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To activate in vivo NK cells, C57BL/6J wild type mice were injected i.p. with a neutralizing rat anti-mouse NKG2A/C/E monoclonal antibody (200g/mouse, BioXCell BE0321, clone 20D5) or with a rat IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Ligation:

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. To activate in vivo NK cells, C57BL/6J wild type mice were injected i.p. with a neutralizing rat anti-mouse NKG2A/C/E monoclonal antibody (200g/mouse, BioXCell BE0321, clone 20D5) or with a rat IgG2a isotype control (200g/mouse BioxCell BE0085 clone 2A3) 1 hour after coronary artery ligation. ..

    Concentration Assay:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: .. A neutralizing anti-PLVAP antibody (clone MECA-32, BioXCell, BE0200) or a rat IgG2a isotype control (BioXCell, BE0089) was diluted to a concentration of 2.11 mg/ml in a 0.9% sodium chloride solution (Millipore Sigma, S8776). .. Osmotic pumps (ALZET, model 2002) were filled with the diluted antibodies according to the manufacturer’s protocol, and then surgically implanted subcutaneously into DIO mice at 20 weeks-of-age.



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    a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
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    a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
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    a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
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    98
    Bio X Cell be0089 bacterial
    a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat <t>IgG2a</t> Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.
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    a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat IgG2a Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.

    Journal: Nature Communications

    Article Title: MALT1 protease inhibition restrains glioblastoma progression by reversing tumor-associated macrophage-dependent immunosuppression in mice

    doi: 10.1038/s41467-026-76572-7

    Figure Lengend Snippet: a Induction of MALT1 protease activity within macrophages was measured using RAW264.7 cells stably expressing a cleavage-activated luciferase reporter, which emits luminescence upon activation of MALT1 protease or b induction of NF-kB activity within macrophages was measured in RAW-Blue macrophages co-cultured with GL261-GBM cells. Results are shown as fold change relative to control macrophages cultured alone. c Cytokines and chemokines in the cell culture supernatant were measured by cytometric bead array 48 h after co-culturing primary mouse macrophages with GL261-GBM cells in the absence or presence of MALT1i. Cytokine levels are presented as a heat map indicating fold change relative to the control macrophages alone. *Significant difference compared to macrophage control alone. #Significant difference compared to macrophage + GBM co-culture. d , e Changes in macrophage M1/M2-like polarization markers after co-culture with GBM cells. d RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of MALT1i or e CARD9i. f NF-kB activity in RAW-Blue macrophages co-cultured with GL261-GBM cells. g Changes in macrophage M2-like polarization markers after co-culture with GBM cells. RAW264.7 mouse macrophages were co-cultured with GL261 mouse GBM cells in the absence or presence of a blocking antibody against mouse Dectin-1 receptor (1–5 µg/mL) or the appropriate Rat IgG2a Isotype Control Antibody (5 µg/mL). h – j The Incucyte live-cell analysis system was used to measure tumor cell apoptosis in real-time. Apoptotic tumor cells are indicated by the overlap (or colocalization) of red and green to produce yellow. h Fluorescent images of RAW264.7 macrophages co-cultured with GBM-GL261 tumor cells ± MALT1i. Scale bar = 400 µM. i Co-cultures of mouse RAW264.7 macrophages with GL261-GBM cells were performed to compare macrophage-dependent tumor cell apoptosis in the absence/presence of MALT1i. j Controls demonstrate that MALT1i has no effect on apoptosis of GL261-GBM tumor cells or RAW264.7 macrophages when either is cultured alone. All values are represented as mean ± SD. Data were analyzed by 1-way ANOVA, followed by Tukey’s multiple-comparisons. P values are indicated in the figure.

    Article Snippet: In experiments with the MALT1 inhibitor, macrophages were exposed to MLT-748 (5 μM, catalog no.: HY-115466, Med Chem Express), BRD5529 (50 μM, catalog no.: HY-115497), antibody against murine Dectin-1 (clone R1-8g7) (1–5 μg/mL, catalog no.: mabg-mdect-2, Invivogen) or the appropriate Rat IgG2a Isotype Control Antibody (catalog no.: mabg2a-ctlrt, Invivogen) for 2 h prior to addition of tumor cells and during the entire co-culture time.

    Techniques: Activity Assay, Stable Transfection, Expressing, Luciferase, Activation Assay, Cell Culture, Control, Co-Culture Assay, Blocking Assay, Cell Analysis